PCMV6-INTS1-3*FLAG
Product Specifications
Applications
Protein Expression
Storage Temperature
37°C
AA Sequence
MNRAKPTTVRRPSAAAKPSGHPPPGDFIALGSKGQANESKTASTLLKPAPSGLPSERKRDAAAALSSASALTGLTKRPKLSSTPPLSALGRLAEAAVAEKRAISPSIKEPSVVPIEVLPTVLLDEIEAAELEGNDDRIEGVLCGAVKQLKVTRAKPDSTLYLSLMYLAKIKPNIFATEGVIEALCSLLRRDASINFKAKGNSLVSVLACNLLMAAYEEDENWPEIFVKVYIEDSLGERIWVDSPHCKTFVDNIQTAFNTRMPPRSVLLQGEAGRVAGDLGAGSSPHPSLTEEEDSQTELLIAEEKLSPEQEGQLMPRYEELAESVEEYVLDMLRDQLNRRQPIDNVSRNLLRLLTSTCGYKEVRLLAVQKLEMWLQNPKLTRPAQDLLMSVCMNCNTHGSEDMDVISHLIKIRLKPKVLLNHFMLCIRELLSAHKDNLGTTIKLVIFNELSSARNPNNMQVLYTALQHSSELAPKFLAMVFQDLLTNKDDYLRASRALLREIIKQTKHEINFQAFCLGLMQERKEPQYLEMEFKERFVVHITDVLAVSMMLGITAQVKEAGIAWDKGEKRNLEVLRSFQNQIAAIQRDAVWWLHTVVPSISKLAPKDYVHCLHKVLFTEQPETYYKWDNWPPESDRNFFLRLCSEVPILEDTLMRILVIGLSRELPLGPADAMELADHLVKRAAAVQADDVEVLKVGRTQLIDAVLNLCTYHHPENIQLPPGYQPPNLAISTLYWKAWPLLLVVAAFNPENIGLAAWEEYPTLKMLMEMVMTNNYSYPPCTLTDEETRTEMLNRELQTAQREKQEILAFEGHLAAASTKQTITESSSLLLSQLTSLDPQGPPRRPPPHILDQVKSLNQSLRLGHLLCRSRNPDFLLHIIQRQASSQSMPWLADLVQSSEGSLDVLPVQCLCEFLLHDAVDDAASGEEDDEGESKEQKAKKRQRQQKQRQLLGRLQDLLLGPKADEQTTCEVLDYFLRRLGSSQVASRVLAMKGLSLVLSEGSLRDGEEKEPPMEEDVGDTDVLQGYQWLLRDLPRLPLFDSVRSTTALALQQAIHMETDPQTISAYLIYLSQHTPVEEQAQHSDLALDVARLVVERSTIMSHLFSKLSPSAASDAVLSALLSIFSRYVRRMRQSKEGEEVYSWSESQDQVFLRWSSGETATMHILVVHAMVILLTLGPPRADDSEFQALLDIWFPEEKPLPTAFLVDTSEEALLLPDWLKLRMIRSEVLRLVDAALQDLEPQQLLLFVQSFGIPVSSMSKLLQFLDQAVAHDPQTLEQNIMDKNYMAHLVEVQHERGASGGQTFHSLLTASLPPRRDSTEAPKPKSSPEQPIGQGRIRVGTQLRVLGPEDDLAGMFLQIFPLSPDPRWQSSSPRPVALALQQALGQELARVVQGSPEVPGITVRVLQALATLLSSPHGGALVMSMHRSHFLACPLLRQLCQYQRCVPQDTGFSSLFLKVLLQMLQWLDSPGVEGGPLRAQLRMLASQASAGRRLSDVRGGLLRLAEALAFRQDLEVVSSTVRAVIATLRSGEQCSVEPDLISKVLQGLIEVRSPHLEELLTAFFSATADAASPFPACKPVVVVSSLLLQEEEPLAGGKPGADGGSLEAVRLGPSSGLLVDWLEMLDPEVVSSCPDLQLRLLFSRRKGKGQAQVPSFRPYLLTLFTHQSSWPTLHQCIRVLLGKSREQRFDPSASLDFLWACIHVPRIWQGRDQRTPQKRREELVLRVQGPELISLVELILAEAETRSQDGDTAACSLIQARLPLLLSCCCGDDESVRKVTEHLSGCIQQWGDSVLGRRCRDLLLQLYLQRPELRVPVPEVLLHSEGAASSSVCKLDGLIHRFITLLADTSDSRALENRGADASMACRKLAVAHPLLLLRHLPMIAALLHGRTHLNFQEFRQQNHLSCFLHVLGLLELLQPHVFRSEHQGALWDCLLSFIRLLLNYRKSSRHLAAFINKFVQFIHKYITYNAPAAISFLQKHADPLHDLSFDNSDLVMLKSLLAGLSLPSRDDRTDRGLDEEGEEESSAGSLPLVSVSLFTPLTAAEMAPYMKRLSRGQTVEDLLEVLSDIDEMSRRRPEILSFFSTNLQRLMSSAEECCRNLAFSLALRSMQNSPSIAAAFLPTFMYCLGSQDFEVVQTALRNLPEYALLCQEHAAVLLHRAFLVGMYGQMDPSAQISEALRILHMEAVM
Reference Sequence ID
NM_001080453.2
Plasmid Hos
Mammalian cell
Inserted Fragment Type
ORF
Inserted Fragment Species
Human
Resistance
Kan
Selection antibiotic
G418
Competent cells
DH5a
Nucleic Acid Sequence
ATGAACCGGGCCAAGCCCACCACGGTGCGCCGGCCCAGCGCCGCGGCCAAACCCTCAGGGCACCCTCCCCCAGGAGACTTCATTGCTCTGGGCTCAAAGGGTCAGGCCAATGAATCGAAAACGGCGTCCACCCTGCTGAAGCCAGCCCCTTCCGGCCTGCCTTCTGAGCGCAAGCGGGATGCGGCGGCCGCGTTGTCCAGTGCCTCGGCCCTCACCGGTCTCACCAAACGCCCCAAACTCTCCTCCACACCCCCTCTGAGTGCCCTGGGGCGCCTGGCTGAGGCTGCAGTGGCAGAAAAACGAGCCATTTCTCCGTCGATTAAAGAGCCATCTGTGGTGCCAATTGAAGTGCTGCCCACGGTGCTGCTGGATGAGATCGAGGCGGCCGAGCTGGAGGGCAACGATGACAGGATCGAGGGCGTGCTGTGCGGGGCCGTGAAGCAGCTGAAGGTCACCCGCGCCAAGCCTGACAGCACCCTCTACCTGAGCCTCATGTACCTGGCCAAGATCAAGCCCAACATCTTCGCCACTGAGGGCGTCATTGAGGCTCTGTGTAGCCTCCTGCGGCGGGACGCCTCCATCAACTTCAAGGCCAAGGGGAACAGCCTGGTGTCTGTGCTGGCCTGTAACCTCCTCATGGCCGCCTACGAGGAGGACGAGAACTGGCCCGAGATCTTTGTCAAGGTGTACATCGAGGACTCCCTGGGGGAGCGGATCTGGGTGGACAGCCCTCACTGTAAGACGTTTGTGGACAACATCCAGACGGCCTTCAACACCAGAATGCCCCCCAGGAGCGTGCTGCTGCAGGGGGAGGCGGGCCGCGTTGCGGGCGACCTGGGTGCAGGGAGCAGCCCACACCCCTCCCTCACGGAGGAGGAGGACAGCCAGACGGAGTTGCTGATCGCGGAGGAGAAGCTGAGCCCCGAGCAGGAGGGCCAGCTCATGCCCAGGTACGAAGAGCTCGCGGAGAGCGTGGAGGAGTATGTCCTGGACATGCTGCGGGACCAGCTGAACCGGCGCCAGCCCATCGACAACGTCTCCAGGAACCTCCTGCGGCTCCTCACCTCCACCTGCGGCTATAAGGAGGTGCGGCTGCTGGCGGTGCAGAAGCTGGAGATGTGGCTGCAGAACCCCAAGCTGACCCGGCCGGCCCAGGACCTGCTGATGTCCGTCTGCATGAACTGCAACACGCACGGTTCCGAAGACATGGACGTCATCTCACACCTGATCAAGATCCGCCTCAAGCCCAAGGTCCTCCTCAACCACTTCATGCTGTGCATCAGGGAGCTGCTGAGCGCGCACAAGGACAACCTGGGCACCACCATCAAGTTGGTGATCTTCAATGAGCTCTCCAGCGCCCGGAACCCGAACAACATGCAGGTCCTCTATACCGCACTGCAGCACAGCTCAGAGCTGGCGCCCAAGTTCCTGGCCATGGTGTTCCAGGACCTGCTGACCAACAAGGACGACTACCTGCGGGCCTCGCGGGCCCTGCTGCGGGAGATCATCAAGCAGACCAAGCACGAGATCAACTTCCAGGCCTTCTGCCTGGGGCTCATGCAGGAGCGCAAGGAGCCGCAGTACCTGGAGATGGAGTTCAAGGAGCGCTTCGTGGTGCACATCACCGACGTCCTGGCCGTGTCCATGATGCTGGGCATCACAGCGCAGGTGAAGGAGGCCGGCATCGCCTGGGACAAAGGAGAAAAGAGGAACCTGGAAGTGCTCCGCTCATTCCAGAACCAGATTGCCGCCATCCAGCGGGATGCCGTCTGGTGGCTCCACACTGTGGTCCCCTCCATCAGCAAGCTCGCCCCTAAGGACTACGTGCACTGCCTGCACAAGGTGCTGTTCACAGAGCAGCCGGAGACCTACTACAAGTGGGACAACTGGCCACCCGAGAGTGACCGCAACTTCTTCCTGCGTCTGTGCTCCGAGGTGCCCATTTTGGAGGACACGCTGATGCGCATCCTGGTCATCGGGCTGTCCCGGGAGCTCCCGCTCGGGCCTGCGGACGCCATGGAGCTTGCTGACCACCTGGTGAAGCGGGCGGCTGCCGTGCAGGCGGATGATGTGGAGGTGCTGAAGGTGGGGAGGACCCAGCTGATCGACGCCGTTCTGAATCTGTGCACCTACCATCACCCTGAAAACATCCAGCTCCCACCGGGGTACCAGCCTCCGAACCTCGCCATCTCTACCCTCTACTGGAAGGCCTGGCCCCTCCTGCTGGTCGTCGCCGCATTCAACCCAGAGAACATCGGCCTGGCTGCGTGGGAGGAGTACCCGACCCTGAAGATGCTCATGGAGATGGTGATGACCAACAACTACTCCTACCCACCGTGCACCCTGACGGATGAGGAGACCCGGACGGAGATGCTGAACCGTGAGCTGCAGACCGCCCAGCGGGAGAAGCAGGAGATCCTGGCCTTCGAGGGGCACCTGGCGGCCGCGTCCACCAAGCAGACCATCACTGAGAGCAGCAGCCTCCTCCTGTCGCAGCTCACCAGCCTGGACCCCCAGGGGCCCCCCCGGAGGCCTCCCCCTCACATCCTGGATCAAGTGAAAAGCCTCAACCAGTCCCTCCGCCTCGGGCACCTCTTGTGCCGCAGCCGAAACCCTGACTTTCTCCTCCACATCATCCAGCGGCAGGCCTCCTCGCAGTCCATGCCCTGGCTGGCGGACCTGGTACAGTCCAGCGAGGGCTCCCTGGACGTGCTGCCCGTGCAGTGTCTGTGCGAGTTCCTGCTGCACGATGCTGTGGACGATGCTGCTTCCGGGGAGGAGGACGACGAGGGCGAGAGCAAGGAGCAGAAGGCCAAGAAGCGGCAGAGGCAACAGAAGCAGCGGCAGCTGCTGGGCCGCCTGCAGGACCTGCTACTGGGCCCGAAGGCTGATGAGCAGACCACGTGTGAGGTGCTGGACTACTTCTTGCGGCGCCTCGGCTCCTCCCAGGTGGCCTCCCGCGTGCTGGCCATGAAGGGTTTGTCGCTGGTGCTTTCGGAGGGCAGCCTGCGGGACGGGGAGGAGAAGGAGCCCCCCATGGAGGAGGATGTGGGGGACACAGATGTGCTGCAGGGCTATCAGTGGCTGCTGCGGGACCTGCCTCGCCTGCCTCTGTTCGACAGCGTCAGGAGCACCACAGCCCTGGCCCTGCAGCAGGCAATCCACATGGAGACTGATCCCCAGACCATCAGCGCCTACCTGATCTACTTGTCCCAGCACACGCCTGTGGAGGAGCAGGCCCAGCACAGCGACCTGGCCCTGGACGTGGCCCGGCTGGTCGTGGAGCGCTCCACCATCATGTCCCACCTCTTCTCGAAGCTCTCCCCGAGTGCCGCGTCGGACGCCGTGCTGAGCGCTCTGTTGTCCATCTTCTCACGCTACGTGAGGCGCATGCGGCAGAGCAAGGAGGGCGAGGAGGTCTACAGCTGGTCGGAGTCTCAGGACCAGGTCTTCCTACGCTGGAGCAGCGGGGAGACAGCCACCATGCACATCCTCGTGGTCCATGCCATGGTGATCCTGCTGACGCTGGGCCCGCCTCGAGCCGACGACAGCGAGTTCCAGGCGCTGCTGGACATCTGGTTTCCGGAGGAGAAGCCACTGCCCACCGCCTTCCTGGTGGACACATCGGAGGAGGCGCTGCTGCTTCCTGACTGGCTGAAGCTGCGCATGATCCGTTCTGAGGTGCTCCGCCTGGTGGACGCCGCCCTGCAGGACCTGGAGCCGCAGCAGCTGCTGCTGTTCGTGCAGTCGTTTGGCATCCCCGTGTCCAGCATGAGCAAACTCCTCCAGTTCCTGGACCAGGCAGTGGCCCACGACCCCCAGACTCTGGAGCAGAACATCATGGACAAGAATTACATGGCCCACCTGGTGGAGGTCCAGCATGAGCGCGGCGCCTCCGGAGGCCAGACTTTCCACTCCTTGCTCACAGCCTCCCTGCCGCCCCGCCGAGACAGCACAGAGGCACCCAAACCAAAGAGCAGCCCAGAGCAGCCCATAGGCCAGGGCCGGATTCGGGTGGGGACCCAGCTCCGGGTGCTGGGCCCTGAGGACGACCTGGCTGGCATGTTCCTCCAGATTTTCCCGCTCAGCCCGGACCCTCGGTGGCAGAGCTCCAGTCCCCGCCCCGTGGCCCTCGCCCTGCAGCAGGCCCTGGGCCAGGAGCTGGCCCGCGTCGTCCAGGGCAGCCCCGAGGTGCCGGGCATCACGGTGCGTGTCCTGCAGGCCCTCGCCACCCTGCTCAGCTCCCCACACGGCGGTGCCCTGGTGATGTCCATGCACCGTAGCCACTTCCTGGCCTGCCCGCTGCTGCGCCAGCTCTGCCAGTACCAGCGCTGTGTGCCACAGGACACCGGCTTCTCCTCGCTCTTCCTGAAGGTGCTCCTGCAGATGCTGCAGTGGCTGGACAGCCCTGGCGTGGAGGGCGGGCCCCTGCGGGCACAGCTCAGGATGCTTGCCAGCCAGGCCTCAGCCGGGCGCAGGCTCAGTGATGTGCGAGGGGGGCTCCTGCGCCTGGCCGAGGCCCTGGCCTTCCGTCAGGACCTGGAGGTGGTCAGCTCCACCGTCCGTGCCGTCATCGCCACCCTGAGGTCTGGGGAGCAGTGCAGCGTGGAGCCGGACCTGATCAGCAAAGTCCTCCAGGGGCTGATCGAGGTGAGGTCCCCCCACCTGGAGGAGCTGCTGACTGCATTCTTCTCTGCCACTGCGGATGCTGCCTCCCCGTTTCCAGCCTGTAAGCCCGTTGTGGTGGTGAGCTCCCTGCTGCTGCAGGAGGAGGAGCCCCTGGCTGGGGGGAAGCCGGGTGCGGACGGTGGCAGCCTGGAGGCCGTGCGGCTGGGGCCCTCGTCAGGCCTCCTAGTGGACTGGCTGGAAATGCTGGACCCCGAGGTGGTCAGCAGCTGCCCCGACCTGCAGCTCAGGCTGCTCTTCTCCCGGAGGAAGGGCAAAGGTCAGGCCCAGGTGCCCTCGTTCCGTCCCTACCTCCTGACCCTCTTCACGCATCAGTCCAGCTGGCCCACACTGCACCAGTGCATCCGAGTCCTGCTGGGCAAGAGCCGGGAACAGAGGTTCGACCCCTCTGCCTCTCTGGACTTCCTCTGGGCCTGCATCCATGTTCCTCGCATCTGGCAGGGGCGGGACCAGCGCACCCCGCAGAAGCGGCGGGAGGAGCTGGTGCTGCGGGTCCAGGGCCCGGAGCTCATCAGCCTGGTGGAGCTGATCCTGGCCGAGGCGGAGACGCGGAGCCAGGACGGGGACACAGCCGCCTGCAGCCTCATCCAGGCCCGGCTGCCCCTGCTGCTCAGCTGCTGCTGTGGGGACGATGAGAGTGTCAGGAAGGTGACGGAGCACCTGTCAGGCTGCATCCAGCAGTGGGGAGACAGCGTGCTGGGCAGGCGCTGCCGAGACCTTCTCCTGCAGCTCTACCTACAGCGGCCGGAGCTGCGGGTGCCCGTGCCTGAGGTCCTACTGCACAGCGAAGGGGCTGCCAGCAGCAGCGTCTGCAAGCTGGACGGACTCATCCACCGCTTCATCACGCTCCTTGCGGACACCAGCGACTCCCGGGCGTTGGAGAACCGAGGGGCGGATGCCAGCATGGCCTGCCGGAAGCTGGCGGTGGCGCACCCGCTGCTGCTGCTCAGGCACCTGCCCATGATCGCGGCGCTCCTGCACGGCCGCACCCACCTCAACTTCCAGGAGTTCCGGCAGCAGAACCACCTGAGCTGCTTCCTGCACGTGCTGGGCCTGCTGGAGCTGCTGCAGCCGCACGTGTTCCGCAGCGAGCACCAGGGGGCGCTGTGGGACTGCCTTCTGTCCTTCATCCGCCTGCTGCTGAATTACAGGAAGTCCTCCCGCCATCTGGCTGCCTTCATCAACAAGTTTGTGCAGTTCATCCATAAGTACATTACCTACAATGCCCCAGCAGCCATCTCCTTCCTGCAGAAGCACGCCGACCCGCTCCACGACCTGTCCTTCGACAACAGTGACCTGGTGATGCTGAAATCCCTCCTTGCAGGGCTCAGCCTGCCCAGCAGGGACGACAGGACCGACCGAGGCCTGGACGAAGAGGGCGAGGAGGAGAGCTCAGCCGGCTCCTTGCCCCTGGTCAGCGTCTCCCTGTTCACCCCTCTGACCGCGGCCGAGATGGCCCCCTACATGAAACGGCTTTCCCGGGGCCAAACGGTGGAGGATCTGCTGGAGGTTCTGAGTGACATAGACGAGATGTCCCGGCGGAGACCCGAGATCCTGAGCTTCTTCTCGACCAACCTGCAGCGGCTGATGAGCTCGGCCGAGGAGTGTTGCCGCAACCTCGCCTTCAGCCTGGCCCTGCGCTCCATGCAGAACAGCCCCAGCATTGCAGCCGCTTTCCTGCCCACGTTCATGTACTGCCTGGGCAGCCAGGACTTTGAGGTGGTGCAGACGGCCCTCCGGAACCTGCCTGAGTACGCTCTCCTGTGCCAAGAGCACGCGGCTGTGCTGCTCCACCGGGCCTTCCTGGTGGGCATGTACGGCCAGATGGACCCCAGCGCGCAGATCTCCGAGGCCCTGAGGATCCTGCATATGGAGGCCGTGATGTGA
Gene Length
6573
Frequently Asked Questions
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