QPCR Lentivirus Titer Kit & ViralEntryTM Bundle

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system functions as a highly versatile and precise tool for genome engineering. It utilizes a guide RNA (gRNA) to direct a nuclease—most commonly Cas9—to a specific target sequence within the genome, where it generates a double-strand break. This mechanism allows for targeted gene disruption (knockout) or precise sequence insertion (knock-in) . This RNA-guided DNA cleavage offers unparalleled simplicity and scalability compared to previous editing technologies. When integrated with viral delivery systems, CRISPR components can be transiently or stably expressed in a wide range of cell types, including primary and non-dividing cells, making it a cornerstone of modern functional genomics and therapeutic development.