2nd Generation Lentivirus Bundle 1 (LV003-Packaging Mix, G2500-Transfection Reagent, LV900-Titer Kit)

This Cas9 Expression Adenoviral System provides a highly efficient method for delivering the Cas9 nuclease into a wide range of mammalian cells. By leveraging the high transduction efficiency of the adenovirus, this system ensures robust Cas9 protein expression, which is essential for successful CRISPR-mediated genome editing. This approach is particularly effective in hard-to-transfect cell lines, primary cells, and non-dividing cells where traditional lipid-based transfection often fails. When used in combination with a guide RNA (gRNA), this system facilitates precise double-strand breaks at target genomic loci, enabling efficient gene knockout or knock-in experiments with minimal toxicity to the host cells.