TO3-3PEG-Biotin Fluorophore

RNA Mango technology is based on the specific binding between the RNA Mango aptamer and a thiazole orange (TO) bi-functional dye. This interaction is characterized by tight binding affinity (KD ≈ 3 nM) and a strong ~1000-fold increase in fluorescence intensity upon binding to the Mango aptamer (fluorescent enhancement FE ≈ 1,100) .