RScript Hot cDNA Synthesis Kit

OneScript® Hot cDNA Synthesis Kit enables highly efficient and robust cDNA synthesis at elevated temperatures (up to 60–72°C), making it especially suitable for difficult RNA templates that are not efficiently reverse transcribed under standard conditions. Unlike conventional kits optimized for 42–55°C, this system uses a thermostable reverse transcriptase derived from Moloney Murine Leukemia Virus (M-MLV RT), engineered to maintain strong activity at higher temperatures. This helps reduce RNA secondary structure issues, improves access to GC-rich regions, and enhances full-length cDNA synthesis. The enzyme is modified to eliminate RNase H activity, preserving RNA integrity during first-strand synthesis and improving yield and fidelity. It also shows improved resistance to inhibitors commonly present in biological samples or RNA extraction reagents. The kit includes abm’s RNaseOFFTM ribonuclease inhibitor, which provides strong RNA protection and remains stable even at very low DTT concentrations (<1 mM), ensuring reliable performance under challenging conditions. Key features: High-temperature cDNA synthesis (60–72°C) for difficult templates Effective on degraded RNA and inhibitor-rich samples High yield from low-abundance RNA Included in the PCR Buffet Program Kit components: OneScript® Hot Reverse Transcriptase (100 reactions / 100 μl) 5X RT Buffer (400 μl) Oligo (dT) (10 μM, 100 μl) Random Primers (10 μM, 100 μl) dNTP (10 mM, 100 μl) Nuclease-free H2O (2 x 1.0 ml) Equivalence: This product is equivalent to RScript Hot cDNA Synthesis Kit (Cat. No. G594EU) . Manufactured under ISO 13485:2016 MDSAP-certified quality management systems.