RScript Hot 5X RT MasterMix

OneScript® Hot 5X RT MasterMix is a ready-to-use solution designed for efficient and reliable first-strand cDNA synthesis. It simplifies workflow while ensuring high reproducibility and strong performance. The master mix contains abm’s OneScript® Hot Reverse Transcriptase, RNaseOFFTM Ribonuclease Inhibitor, dNTPs, and a balanced combination of oligo (dT) and random primers. The reverse transcriptase is engineered with modifications that eliminate RNase H activity, improving thermal stability and preserving RNA integrity during reverse transcription, which results in higher cDNA yield and quality. This formulation is ideal for generating high-quality cDNA suitable for a wide range of downstream molecular biology applications. Key features: Simple one-step setup with reduced pipetting and handling High cDNA yield, even from difficult RNA samples Strong protection against RNase contamination via RNaseOFFTM Includes dNTPs, oligo (dT), and random primers Includes OneScript® Hot Reverse Transcriptase (Cat. No. G593) Includes RNaseOFFTM inhibitor (Cat. No. G591) Included in the PCR Buffet Program Kit components: OneScript® Hot 5X RT MasterMix (100 reactions / 400 μl) Nuclease-free H2O (1.0 ml) Equivalence: This product is equivalent to RScript Hot 5X RT MasterMix (Cat. No. G590EU) . Manufactured under ISO 13485:2016 MDSAP-certified quality management systems.