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Anti-Histone H4 tetra acetyl Antibody

Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.

Product Specifications

Host

Rabbit

Species Reactivity

Human

Immunogen

Peptide containing the amino terminal region of Tetrahymena histone variant hv1 acetylated at multiple lysines.

Clonality

Polyclonal

Isotype

IgG

Type

Polyclonal Antibodies

Applications

WB

Concentration

1 μg/μL

Purity

Purified

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Specificity

The immunogen has a high degree of homology to histone H4, so the antibody primarily recognizes tetra-acetylated histone H4, secondarily recognizes tri-acetylated forms of histone H4. Faint recognition of multiply acetylated histone H2A is observed.

Applications Notes

The sequence of the immunogen from Tetrahymena hv1 has a high degree of homology to histone H4 in higher organisms. This modification is conserved from yeast to human and a wide range of reactivity is expected.

Storage Buffer

PBS, pH 7.4 with 0.05% sodium azide.

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