Cell-Free Protein Synthesis Kit (pAzF)
Cell-Free Protein Synthesis Kit is an extract-based transcription/translation system derived from E. coli cells. It enables the site-specific incorporation of the noncanonical amino acid p-acetyl-L-phenylalanine (pAcF) into the target sequence by utilizing the amber stop codon TAG in the DNA sequence. The kit contains three additional components: the noncanonical amino acid pAcF, tRNA, and aminoacyl-tRNA synthetase. Dosage can be optimized according to requirements. Note: This product under this catalog number enables the expression of disulfide bond-rich proteins.
Product Specifications
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/11danbaiyanjiuxilie/EG25331.pdf
Components
• Cell-free system solution A: 300 μl • Cell-free system solution B: 600 μl • CFPS-Control Plasmid: 2 μg • Noncanonical amino acid pAcF (100x) : 50 μl • tRNA (25x) : 100 μl • Aminoacyl-tRNA synthetase (50x) : 50 μl
Storage Conditions
Store at -80°C. Valid for 12 months. Dry ice transportation. After opening, all components should be stored at -80°C. To avoid multiple freeze-thaw cycles, Solution A and Solution B can be aliquoted according to reaction volume, snap-frozen in liquid nitrogen, and then stored at -80°C.
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