What is the catalog number for ApeKI?

The catalog number for ApeKI is EG25519S.

What sizes are available for ApeKI?

ApeKI is available in the following size(s): 500 U.

ApeKI is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures ApeKI?

ApeKI is manufactured by Cleavion.

What is the estimated delivery time for ApeKI?

Estimated delivery time for ApeKI is approximately 35 business day(s), depending on your location.

ApeKI

ApeKI is a Type IIP restriction enzyme derived from the Aeropyrum pernix K1. It recognizes and cleaves the sequence G/CWGC, generating sticky-end with a 3-base overhang. ApeKI is one of the preferred enzymes for Genotyping-by-Sequencing (GBS) and is widely used in plant and animal genetics, population evolution studies, and functional gene mapping.

Product Specifications

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/EG25519.pdf

Components

• ApeKI (10 U/μl) : 50 μl • 10x Cut Buffer C: 1 ml

Features & Benefits

Recommended Reaction Conditions 1x Cut Buffer C; Incubate at 75°C; Refer to "Protocol for DNA Digestion" for reaction setup. Heat Inactivation This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.