TRUPCR® EGFR Real-time PCR Kit
• CE-IVD • Selective amplification of DNA containing mutations with ARMS Technology. • Sensitive to detect up to 1% mutation in EGFR gene. • Detects 32 different mutations in a single run. • Extraction control included to avoid false-negative results. • Rapid, more reliable, comprehensive and cost-effective tests. • Easy workflow & compatible with various Real-time PCR instruments.
Product Specifications
Applications Notes
The TRUPCR® EGFR Real-time PCR Kit is an in vitro diagnostic test intended for the qualitative detection of EGFR somatic mutations in genomic DNA extracted from fresh, frozen or formalin-fixed paraffin-embedded (FFPE) tissue. This real-time EGFR PCR kit is based on allele-specific amplification and is achieved by ARMS PCR. Taq DNA polymerase is extremely effective at distinguishing between a match and a mismatch at the 3’-end of a PCR primer. The kit is designed to selectively amplify mutant-specific sequences in samples that contain a mixture of wild-type and mutated DNA. The most common mutations are found in exons 19 and 21. The detection is achieved using fluorescent probes labelled with FAM and HEX. The TRUPCR® EGFR PCR Kit is composed of 9 assays for the detection of the EGFR mutations and a reference control gene of the EGFR region without any known polymorphisms/ mutations.
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