TRUPCR® JAK2 Mutation Detection Kit

TRUPCR® JAK2 Mutation Detection Kit (V2.0) is a Real-time polymerase chain reaction (PCR) assay for qualitative detection of V617F mutation in JAK2 (Janus Kinase 2) gene against a background of wild-type genomic DNA. This mutation is a guanine to thymidine transversion in position 1849 of the JAK2 gene, which leads to a valine to phenylalanine substitution in position 617 of the protein (V617F) . The TRUPCR® JAK2 Mutation Mutation Kit is based on ARMS PCR. Taq DNA polymerase is extremely effective at distinguishing between a match and a mismatch at the 3’-end of a PCR primer. The kit is designed to selectively amplify mutant-specific sequences in samples that contain a mixture of wild-type and mutated DNA.