Human Klotho beta / KLB Protein, His Tag

KLB (Klotho Beta) is a Protein Coding gene. Among its related pathways are RET signaling and HIV Life Cycle. GO annotations related to this gene include hydrolase activity, hydrolyzing O-glycosyl compounds and fibroblast growth factor binding. An important paralog of this gene is KL. Klotho Beta is a regulator in multiple metabolic processes, while its role in cancer remains unclear. We found the expression of βKlotho was down-regulated in human hepatocellular carcinoma tissues compared with that in paired adjacent non-tumourous liver tissues. Hepatoma cells also showed decreased expression of βKlotho compared with normal hepatocyte cells. Reintroduction of βKlotho into hepatoma cells inhibited their proliferation.

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 112.9 kDa. The protein migrates as 120-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Immobilized Human Klotho beta Protein, His Tag (Cat. No. KLB-H52H4) at 10 μg/mL (100 μL/well) can bind Human FGF19, Fc Tag (Cat. No. FG9-H5253) with a linear range of 0.005-0.156 μg/mL (QC tested).