Osteopontin Recombinant Rabbit Monoclonal Antibody [PSH09-24]
Osteopontin (OPN), also known as bone /sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. The prefix osteo- indicates that the protein is expressed in bone, although it is also expressed in other tissues. The suffix -pontin is derived from "pons," the Latin word for bridge, and signifies osteopontin's role as a linking protein. Osteopontin is an extracellular structural protein and therefore an organic component of bone.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Osteopontin Bone sialoprotein 1 Nephropontin Secreted phosphoprotein 1 (SPP-1) Urinary stone protein Uropontin SPP1 BNSP, OPN PSEC0156
Swiss Prot
P10451 Human, P10923 Mouse
Cellular Locus
Secreted.
Host
Rabbit
Species Reactivity
Human,Mouse
Immunogen
Recombinant protein within human Osteopontin aa 1-314.
Isotype
IgG
Clone
PSH09-24
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, FC
Positive Control
RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for last 3 hours cell lysate, A549 cell lysate, U-87 MG cell lysate, U-87 MG, A549.
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IF-Cell: 1:1,000-1:20,000 ;FC: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 35 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
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