SiRNA Negative Control

Negative Control Duplexes are ideal for use in RNA interference (RNAi) experiments as a control for sequence independent effects following siRNA delivery in any vertebrate cell line.

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.