MGMT siRNA (Rat)
SiRNA to inhibit MGMT expression using RNA interference
Product Specifications
Product Name Alternative
Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Reactivity
R
Source
Synthetic
Applications
RNAi
Purity
> 97%
Form
Lyophilized powder
Components
We offers pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MGMT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot.
Storage Conditions
Shipped at 4 °C. Store at -20 °C for one year.
Specificity
MGMT siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
NCBI Gene Symbol
MGMT
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
SwissProt (Rat)
P24528
Entrez Gene Rat
25332
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