GLI3 siRNA (Mouse)

SiRNA to inhibit GLI3 expression using RNA interference

Product Specifications

Product Name Alternative

Transcriptional activator GLI3; GLI3 form of 190 kDa; GLI3-190; GLI3 full length protein; GLI3FL

Reactivity

Source

Synthetic

Applications

RNAi

Purity

> 97%

Form

Lyophilized powder

Components

We offers pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GLI3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot.

Storage Conditions

Shipped at 4 °C. Store at -20 °C for one year.

Specificity

GLI3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

NCBI Gene Symbol

GLI3

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.