PDE5A siRNA (Human)
SiRNA to inhibit PDE5A expression using RNA interference
Product Specifications
Product Name Alternative
PDE5; cGMP-specific 3'.5'-cyclic phosphodiesterase; cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE
Reactivity
H
Source
Synthetic
Applications
RNAi
Purity
> 97%
Form
Lyophilized powder
Components
We offers pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PDE5A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot.
Storage Conditions
Shipped at 4 °C. Store at -20 °C for one year.
Specificity
PDE5A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
NCBI Gene Symbol
PDE5A
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
SwissProt (Human)
O76074
Entrez GeneHuman
8654
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