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Anti-Hsp70/Hsc70 (plant) Antibody

Mouse Monoclonal Antibody specific to Hsp70/Hsc70 (Plant)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Hsp70/Hsc70 (Plant) Mouse Monoclonal Antibody

Host

Mouse

Reactivity

Plant

Immunogen

Purified Hsp70 from Phaseolus aureus (mung bean).

Target

Hsp70/Hsc70 (plant)

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~70kDa is detected <br><br>ELISA: use at 1-10ug/mL. <br><br>These are recommended concentrations; <br><br>Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes constitutive and inducible plant Hsp70. It does not cross-react with human, rat, bacteria (DNAKK) or human BIP.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Hsp70 genes encode heat-inducible 70kDa heat shock proteins. Hsp70 binds ATP with high affinity and possesses weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. The structure of the ATP binding domain displays multiple features of nucleotide binding proteins. The binding of ATP triggers a critical conformational change which leads to the release of the bound substrate protein. The universal ability of Hsp70s to undergo cycles of binding to and release from partially unfolded proteins is the basis of their role in protein synthesis, protein folding, oligomerization, and protein transport.

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