Anti-His-tag Antibody - AcalephFluor532

KLH-conjugated synthetic peptide encompassing a sequence of His-tag.

Monoclonal

AcalephFluor532

Mouse

WB, IF, FC

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

WB (1/2000 - 1/5000), IF (1/500 - 1/2000), FC (1/1000 - 1/4000)

1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Recognizes C-terminal, internal, and N-terminal His-tag fusion proteins.

Ex/Em = 525/554 nm