Horseradish Peroxidase Chicken Anti-Rabbit IgG Secondary Antibody
Our Horseradish Peroxidase Chicken Anti-Rabbit IgG secondary antibody from Aves Labs is produced in-house. It detects rabbit IgG and is affinity purified.
Product Specifications
Host
Chicken
Species Reactivity
Rabbit
Immunogen
IgG
Target
Rabbit IgG
Clonality
Polyclonal
Isotype
IgY
Type
Secondary Antibody
Applications
IHC, ICC, WB
Concentration
1 mg/mL
Format
Affinity Purified
Buffer
HEPES (50 mM, pH 7.0), NaCl (0.1 M), bovine serum albumin (0.2%, w/v), thimerosal (0.01%, w/v) (as an anti-microbial agent)
Shipping Conditions
Ice packs
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Notes
Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains) . Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-rabbit IgG’s were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains) and then conjugated with horseradish peroxidase.
Specificity
H&L; there is no detectable cross-reactivity with goat, human or mouse IgG.
Quality Control
Antibodies were tested for specificity using double-immunodiffusion in agarose gels and ELISA analysis. In the latter, a 1:20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using tetramethylbenzidine as substrate.
Recommended Usage
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Physical Properties
Liquid
Immunogen Species
Rabbit
Conjugate
HRP
RRID
AB_2313530
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