Fluoresceinated Chicken Anti-Rabbit IgG Secondary Antibody

Our Fluoresceinated Chicken Anti-Rabbit IgG secondary antibody from Aves Labs is produced in-house. It detects rabbit IgG and is affinity purified.

Product Specifications

Host

Chicken

Species Reactivity

Rabbit

Immunogen

IgG

Target

Rabbit IgG

Clonality

Polyclonal

Isotype

IgY

Type

Secondary Antibody

Applications

IHC, ICC, WB

Concentration

1 mg/mL

Format

Affinity Purified

Buffer

Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent)

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains) . Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG’s were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains) and then conjugated with fluorescein isothiocyanate.

Specificity

H&L; there is no detectable cross-reactivity with goat, human or mouse IgG.

Quality Control

Antibodies were tested for specificity using double-immunodiffusion in agarose and for non-specific binding using cryostat sections of unfixed embryonic chick tissues.