Fluoresceinated Goat Anti-Chicken IgY Secondary Antibody
Our Fluoresceinated Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.
Product Specifications
Host
Goat
Species Reactivity
Chicken
Immunogen
IgY
Target
Chicken IgY
Clonality
Polyclonal
Isotype
IgG
Type
Secondary Antibody
Applications
IHC, ICC, WB
Concentration
1 mg/mL
Format
Affinity Purified
Buffer
Phosphate-buffered saline (PBS) (pH 7.2), bovine serum albumin (0.2%, w/v), sodium azide (0.02%, w/v) (as an anti-microbial agent)
Shipping Conditions
Ice packs
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Notes
Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to fluorescein isothiocyanate.
IHC Dilution
1:500-1:1000
Specificity
H&L; does not cross-react with mammalian IgGs.
ICC Dilution
1:500-1:1000
Quality Control
Antibodies were analyzed using immunoelectrophoresis and immunocytochemistry. In this latter test, abundant fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1:10 dilution in PBS), incubation with chicken anti-vimentin antibodies (1:1000 dilution in PBS) and then incubation with GGHL-30F-2 (1:200 dilution in PBS) .
Recommended Usage
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Physical Properties
Liquid
Immunogen Species
Chicken
Conjugate
FITC
RRID
AB_2313516
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