Biotinylated Goat Anti-Chicken IgY Secondary Antibody
Our Biotinylated Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.
Product Specifications
Host
Goat
Species Reactivity
Chicken
Immunogen
IgY
Target
Chicken IgY
Clonality
Polyclonal
Isotype
IgG
Type
Secondary Antibody
Applications
IHC, ICC, WB
Concentration
1 mg/mL
Format
Affinity Purified
Buffer
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), sodium azide (0.02%, w/v) (as an anti- microbial)
Shipping Conditions
Ice packs
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Notes
Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to biotin via a 6-carbon spacer.
Specificity
H&L; does not cross-react with mammalian IgGs.
Quality Control
Antibodies were analyzed using immunoelectro-phoresis and a sandwich ELISA using HRP-labeled streptavidin. In this latter test, a 1:500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with tetramethylbenzidine as the substrate.
Recommended Usage
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Physical Properties
Liquid
Immunogen Species
Chicken
Conjugate
Biotin
RRID
AB_2313506
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