Anti-Rab 9 (clone 9) Recombinant Chicken Chimeric mAb

Rab9 is a small GTP-binding protein of the Rab family that regulates late endosomal trafficking. It is primarily involved in the transport of mannose-6-phosphate receptor-containing vesicles from late endosomes back to the trans-Golgi network, a pathway critical for proper lysosomal enzyme sorting and recycling. Like other Rab proteins, Rab9 functions as a molecular switch, cycling between an active GTP-bound state and an inactive GDP-bound state, with its activity controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs) . Rab9-mediated trafficking ensures the steady-state distribution of cation-dependent and cation-independent mannose-6-phosphate receptors and contributes to lysosomal homeostasis. Dysregulation of Rab9 function has been linked to impaired lysosomal degradation, altered receptor recycling, and has emerging associations with cancer progression, viral infection, and lysosomal storage disorders.

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone Rab9, clone 9 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone Rab9, clone 9 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when tested by Western Blot.