Anti-Cortactin (4F11-G5-F10) Recombinant Chicken Chimeric mAb

Cortactin is an ~80–85 kDa actin-binding protein that orchestrates cytoskeletal remodeling and signal integration. It contains an N-terminal repeat domain that associates with filamentous actin, followed by a helical segment, a proline-rich region with multiple signaling motifs, and a C-terminal SH3 domain. Through interactions with the Arp2/3 complex and other regulators, cortactin promotes actin branching, lamellipodia formation, and endocytic trafficking. Tyrosine phosphorylation by Src family kinases inhibits its actin crosslinking activity, linking receptor-mediated signals to cytoskeletal rearrangements. Aberrant cortactin activity has been implicated in tumor cell invasion, metastasis, and vascular permeability disorders, highlighting its importance in both normal cellular dynamics and disease progression.

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone 4F11-G5-F10 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone 4F11-G5-F10 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when tested by Western Blot.