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Anti-VAChT (N425/45) Recombinant Chicken Chimeric mAb FL650 Conjugate

Selected Variant

78-429-FL650

Size: 200 µL

Our Anti-VAChT chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N425/45. It detects mouse and rat VAChT, and is purified by affinity chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Vesicular acetylcholine transporter (VaCht), or Solute Carrier Family 18 Member A3 is encoded by the gene Slc18a3 and is a member of the vesicular amine transporter family. VaCht transports acetylcholine into secretory vesicles for release into the extracellular space. VaCht is expressed in major cholinergic cell groups in the peripheral and central cholinergic nervous systems. Diseases associated with SLC18A3 include Myasthenic Syndrome and Presynaptic and Presynaptic Congenital Myasthenic Syndromes.

Synonyms

Vesicular acetylcholine transporter (VAChT) (rVAT) (Solute carrier family 18 member 3)

Gene Name

Slc18a3 Vacht Vat

UniProt

Q62666

Host

Chicken

Species Reactivity

Mouse, Rat

Immunogen

Fusion protein amino acids 55-125 (first vesicle lumen domain) of rat VAChT (accession number Q62666) produced recombinantly in E. Coli

Target

VAChT

Clonality

Recombinant

Isotype

IgY

Clone

N425/45

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Transporter Proteins

Concentration

0.5 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N425/45 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N425/45 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

WB Dilution

Q9BXM7

Specificity

Does not cross-react with VMAT1 or VMAT2

Quality Control

Q9BXM7

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

IHC Quality Control

1

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