Anti-Beta4-Spectrin (N393/76) Recombinant Chicken Chimeric mAb

Our chicken Anti-Beta4-spectrin recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N393/76. It detects human, mouse, and rat Beta4-spectrin and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Product Specifications

Background

Spectrin beta chain, non-erythrocytic 4, or ß4 spectrin, is a member of the spectrin family of cytoskeletal proteins. It is found in the cytoskeleton and cell cortex of cells in the brain and pancreatic islets. It is also expressed in skeletal muscule. ß4 spectrin functions as a scaffold protein that links the actin cytoskeleton to the plasma membrane. It is involved in cell shape and organization. Mutations in the ß4 spectrin gene are associated with congenital myopathy, and neurodevelopmental disorder with hypotonia, neuropathy and deafness (NEDHND) .

Synonyms

Spectrin beta chain, non-erythrocytic 4 (Beta-IV spectrin) (Spectrin, non-erythroid beta chain 3)

Gene Name

SPTBN4 KIAA1642 SPTBN3

UniProt

Q9H254

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (accession number Q9H254) produced recombinantly in E. Coli

Target

Beta4-spectrin

Clonality

Recombinant

Isotype

IgY

Clone

N393/76

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Markers / Neuronal Markers / Axonal Markers / Scaffold Proteins

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

>200 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N393/76 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N393/76 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.