Anti-Olig1 (N149/25) Recombinant Chicken Chimeric mAb

Our Anti-Olig1 chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N149/25. It detects mouse Olig1, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC, ELISA.

Product Specifications

Background

Oligodendrocyte transcription factor 1, Basic Domain, Helix-Loop-Helix Protein, Class B, 6 or Class E Basic Helix-Loop-Helix Protein 21 is encoded by the gene Olig1. Olig1 is a transcription factor and a member of the basic helix-loop-helix (bHLH) factor family. Olig1 promotes formation and maturation of oligodendrocytes (oligodendrogensis) . Olig1 is expressed in spinal cord and brain during development, and by oligodendrocytes after late embryonic development. Olig1 is a useful marker for Oligodendrocyte staining. Diseases associated with this gene include Oligodendroglioma and Grade Iii Astrocytoma.

Synonyms

Oligodendrocyte transcription factor 1 (Oligo1) (Oligodendrocyte-specific bHLH transcription factor 1)

Gene Name

Olig1

UniProt

Q9JKN5

Host

Chicken

Species Reactivity

Mouse

Immunogen

Fusion protein amino acids 1-67 (N-terminus) of mouse Olig1 (accession number Q9JKN5) produced recombinantly in E. Coli

Target

Olig1

Clonality

Recombinant

Isotype

IgY

Clone

N149/25

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Markers / Glial and Schwann Cell Markers / Oligodendrocyte Markers / Neurogenesis and Development / Transcription Regulation

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

30 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N149/25 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N149/25 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.