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Anti-VGlut2 (N29/29) Recombinant Chicken Chimeric mAb FL650 Conjugate

Our chicken Anti-VGlut2 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N29/29. It detects electric ray, human, mouse and rat VGlut2, and is purified by affinity chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Vesicular glutamate transporter 2 (Vglut2) is encoded by the gene Slc17a6 and belongs to the vesicular glutamate transporter family. Vglut2 is expressed in the thalamus, midbrain, brainstem, and spinal cord at synaptic vesicles. It functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut2 has been implicate in include Gnathodiaphyseal Dysplasia and tendinosis.

Synonyms

Vesicular glutamate transporter 2 (VGluT2) (Differentiation-associated BNPI) (Differentiation-associated Na (+) -dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 6)

Gene Name

Slc17a6 Dnpi Vglut2

UniProt

Q9JI12

Host

Chicken

Species Reactivity

Chicken,Electric Ray,Human,Mouse,Rat

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2 (accession number Q9JI12) produced recombinantly in E. Coli

Target

VGlut2

Clonality

Recombinant

Isotype

IgY

Clone

N29/29

Type

Primary Antibody

Applications

ICC, IHC

Field of Research

Cell Markers / Neuronal Markers / Synapse Markers / Pre-synaptic Markers / Transporter Proteins

Concentration

0.5 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N29/29 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N29/29 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

IHC Dilution

1:100-1:1000

Specificity

Does not cross-react with VGlut1 or VGlut3

ICC Dilution

1:100-1:1000

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

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