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Anti-Pink1 PTEN Induced Putative Kinase 1 (N4/15) Recombinant Chicken Chimeric mAb FL594 Conjugate

Selected Variant

78-060-FL594

Size: 200 µL

Our Anti-Pink1 PTEN induced putative kinase 1 chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N4/15. It detects human, mouse, and rat Pink1 PTEN induced putative kinase 1, and is purified by affinity chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

PTEN Induced Kinase 1 is encoded by the gene PINK1. PINK1 is a member of the protein kinase superfamily, Ser/Thr protein kinase family. PINK1 is a serine/threonine protein kinase that protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. PINK1 is highly expressed in the heart, skeletal muscle, and testis. Diseases associated with PINK1 include Parkinson Disease 6, Autosomal Recessive Early-Onset and Parkinson Disease 2, Autosomal Recessive Juvenile.

Synonyms

Serine/threonine-protein kinase PINK1, mitochondrial (EC 2.7.11.1) (BRPK) (PTEN-induced putative kinase protein 1)

Gene Name

PINK1

UniProt

Q9BXM7

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 112-496 of human Pink1 (accession number NP_115785) produced recombinantly in E. Coli

Target

Pink1 PTEN induced putative kinase 1

Clonality

Recombinant

Isotype

IgY

Clone

N4/15

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Mitochondrial Biology / Neurodegenerative Disease / Parkinson's Disease

Concentration

0.5 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

63 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N4/15 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N4/15 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

IHC Dilution

1:200-1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:200-1:1000

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when tested by Western Blot.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

ICC Quality Control

1

Conjugate

FL594 Ex: 594 nm, Em: 615 nm

Price

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