What is the catalog number for Anti-V5 Antibody?

The catalog number for Anti-V5 Antibody is ET-V5-0020.

What sizes are available for Anti-V5 Antibody?

Anti-V5 Antibody is available in the following size(s): 100 µL.

Is Anti-V5 Antibody in stock?

Anti-V5 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-V5 Antibody

Our Anti-V5 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects V5, and is affinity purified. It is great for use in ICC, WB.

Product Specifications

Background

The V5 tag is derived from a protein epitope found in the P and V proteins of paramyxovirus from the simian virus 5 family. The V5 tag is generally added as an epitope tag to proteins using all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence. Proteins tagged with the V5 epitope tag are detected with high sensitivity and specificity using our chicken anti-V5 antibody using various applications including western blot, immunocytochemistry, and immunohistochemistry.

Host

Chicken

Immunogen

Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein

Target

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

ICC, WB

Field of Research

Anti-Tag Antibodies

Concentration

0.2 mg/mL

Format

Affinity purified

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

WB Dilution

1:500-1:1000

ICC Dilution

1:100-1:500

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on a V5 overexpression cell lysate.