Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-Prostatic Acid Phosphatase (PAP) Antibody

Recombinant mouse PAP protein was expressed in bacteria. Antibodies against this protein were prepared by injecting purified recombinant PAP into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Background

Mouse Prostatic Acid Phosphatase (PAP) is a 43,698 dalton protein (381 amino acids; NCBI accession number AAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.

Synonyms

Prostatic acid phosphatase, PAP, EC 3.1.3.2., 5'-nucleotidase, 5'-NT, EC 3.1.3.5, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase, [Cleaved into: PAPf39]

Gene Name

ACPP

UniProt

P15309

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Recombinant mouse PAP expressed in Escherichia coli

Target

Prostatic Acid Phosphatase (PAP)

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

IHC, ICC, WB

Field of Research

Cell Markers / Neuronal Markers

Concentration

10 mg/mL

Format

Mixture of IgY fraction and affinity-purified antibodies

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Molecular Weight

45 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Notes

Chickens were immunized with recombinant mouse Prostatic Acid Phosphatase protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using the PAP protein conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 μg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

IHC Dilution

1:500-1:1000

WB Dilution

1:1000-1:2000

ICC Dilution

1:500-1:1000

Quality Control

Antibodies were analyzed using immunohistochemistry with tissue sections through a 10%-formalin fixed adult mouse. Sections were examined for PAPpositive dorsal root ganglion sensory neurons. Secondary antibodies (fluorescein-labeled goat anti-chicken IgY, Aves Cat. #F-1004) were used at a concentration of 1:500.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

Conjugate

Unconjugated

RRID

AB_2313557

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

CD40L (CD40LG) Rabbit Monoclonal Antibody [Clone ID: AT161-10]
TA386127 200 µg

CD40L (CD40LG) Rabbit Monoclonal Antibody [Clone ID: AT161-10]

Sign In for Pricing
View Details
Cyclin B1 (V92.1), CF740 conjugate, 0.1mg/mL
BNC740680-500 1x 500 µL

Cyclin B1 (V92.1), CF740 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
(2R)-4-Phenylbutan-2-amine-d3
TRC-P319262-10MG 10 mg

(2R)-4-Phenylbutan-2-amine-d3

Sign In for Pricing
View Details
DNA2 Human Gene Knockout Kit (CRISPR)
KN417395 1 Kit

DNA2 Human Gene Knockout Kit (CRISPR)

Sign In for Pricing
View Details
Malt1 (NM_172833) Mouse Over-expression Lysate
LY724110 100 µg

Malt1 (NM_172833) Mouse Over-expression Lysate

Sign In for Pricing
View Details
Di-tert-butyl Peroxide
TRC-D495293-1ML 1 mL

Di-tert-butyl Peroxide

Sign In for Pricing
View Details