Anti-Olig2

Our Anti-Olig2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Olig2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.

Product Specifications

Background

OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) is a basic helix-loop-helix (bHLH) transcription factor essential for oligodendrocyte and motoneuron development in the spinal cord (Ono et al., 2008) . OLIG2 is also known to play a key role in mediating the brains response to cortical injury through post-injury gliosis (Chen Y et al., 2008) and is also highly expressed in malignant glioma where it has been implicated in glioma progression.

Synonyms

BHLHB1, BHLHE19, PRKCBP2, RACK17

Gene Name

OLIG2

UniProt

Q13516

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

N-terminal half of human OLIG2 was used to immunize chickens to avoid cross-reactivity with OLIG3.

Target

Olig2

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

IHC, ICC, WB

Field of Research

Cell Markers / Glial and Schwann Cell Markers / Neurogenesis and Development / Transcription Regulation

Concentration

0.2 mg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered saline (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

35 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

IHC Dilution

1:1000

WB Dilution

1:1000

ICC Dilution

1:1000

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from cells expressing Olig2.