Anti-NFL (Neurofilament Light Chain) Antibody

Three antipeptide antibodies were generated in chickens against sequences shared between the mouse (P08551) and human (P07196) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Background

Human Neurofilament Light Chain (NFL) is a 61,516 dalton protein (543 amino acids) expressed in the axons of CNS and PNS neurons, where it plays a role in maintaining the structural integrity of the axon. Mutations of the NFL gene product are responsible for some forms of autosomal dominant Charcot-Marie-Tooth neuropathies in humans (i.e., CMT type 1F) .

Synonyms

Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

Gene Name

NEFL NF68 NFL

UniProt

P07196

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptides

Target

Neurofilament Light Chain (NFL)

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

IHC, ICC, WB

Field of Research

Cell Markers / Neuronal Markers / Axonal Markers /Neurodegenerative Disease

Concentration

300 μg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

70 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NF-L gene product, but are shared between the human (P07196, NCBI) and mouse (P08551, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 μg/mL. Finally, equal volumes of each of the three affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

IHC Dilution

1:1000-1:2000

WB Dilution

1:1000-1:2000

ICC Dilution

1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.