Anti-Neu-N (Fox 3) Antibody

Our Anti-Neu-N primary antibody from Aves Labs is chicken polyclonal. It detects human, mouse, and rat Neu-N and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Human Neu-N (Fox 3) is a 33,873 dalton (312 amino acid) RNA-binding protein. This protein is found in the nuclei of virtually all post-mitotic central and peripheral neurons and has been used extensively as a general neuronal marker. In these studies, Neu-N (Fox 3) stains neuronal nuclei relatively uniformly, except in the region of the nucleolus, providing a distinctive donut (i.e., torus) shape. Since this antigen is not seen in the cytoplasm, it offers the advantage of being useful in co-immunostaining studies with rabbit and mouse antibodies against various cytoplasmic neuronal antigens.

Synonyms

RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen)

Gene Name

RBFOX3

UniProt

A6NFN3

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

N-terminal 100 amino acids of human FOX3 expressed in and purified from E. coli

Target

Neu-N (Fox 3)

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

ICC, IHC, WB

Field of Research

Cell Markers / Neuronal Markers

Concentration

Lot dependent

Format

Total IgY fraction

Buffer

Concentrated IgY preparation plus 0.02% NaN3

Molecular Weight

46, 48 kDa

Shipping Conditions

Ice packs

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Notes

Total IgY fraction

IHC Dilution

1:2000 - 1:4000

WB Dilution

1:250-1:500

Specificity

Does not cross react with Fox1 or Fox2

ICC Dilution

1:2000-1:4000

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Dilution

1:250-1:500

Conjugate

Unconjugated

IHC Quality Control

Curated Selection

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