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Anti-mScarlet3 Antibody

Our Anti-mScarlet3 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects mScarlet3, and is affinity-purified IgY. It is great for use in ICC, IHC, WB.

Product Specifications

Background

MScarlet3 is a next-generation red fluorescent protein (RFP) widely used in cellular and molecular biology for live-cell imaging. It is part of the mScarlet series, developed to improve brightness, photostability, tolerance to acidity, and maturation speed over previous RFPs (Bindels et al 2017) . mScarlet3 introduces enhancements like better acid tolerance and increased brightness, enabling the capture of clearer, precise, long-term images of live cells. These improvements are especially valuable in studying dynamic cellular processes, such as protein expression and cell signaling where prolonged and high-resolution imaging is essential. mScarlet3 enables the tracking of protein expression, cell signaling, and subcellular dynamics with greater fidelity and less cellular stress.

Host

Chicken

Immunogen

Purified recombinant mScarlet3 expressed in E.Coli

Target

MScarlet3

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

ICC, IHC, WB

Field of Research

Anti-Tag Antibodies

Concentration

200 μg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

26 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

WB Dilution

1:500-1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:50-1:200

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

WB Quality Control

1

Trial Size

1

Conjugate

Unconjugated

RRID

AB_3665368

Frequently Asked Questions

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