Anti-mKate2 Antibody

Our Anti-mKate2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects mKate2, mRuby, TurboRFP and other fluorescent proteins derived from Entacmaea quadricolor. It is affinity-purified IgY and is great for use in ICC, IHC, WB.

Product Specifications

Background

MKate2 is a red fluorescent protein derived from Entacmaea quadricolor, a Bubble-tip anemone native to the tropical waters of the Indo-Pacific region. mKate2 is a rapidly maturing, monomeric fluorescent protein with excitation at 588nm and emission at 633nm and hence is considered a 'far-red' fluorescent protein. Due to its far-red emission mKate2 is particularly well suited to in vivo imaging and it is also known to be brighter and more photostable than the popular fluorescent protein mCherry. Related fluorescent proteins derived from Entacmaea quadricolor include mRuby, mTagBFP2, TurboRFP, mCardinal, and TagRFP-T. Because of the high sequence conservation between these highly-related fluorescent proteins our chicken anti-mKate2 polyclonal antibody recognizes each of these fluorescent proteins allowing you the flexibility to stain your samples for mKate2, mRuby, mTagBFP2, TurboRFP, mCardinal, or TagRFP-T with a single reagent.

Host

Chicken

Immunogen

Purified recombinant mKate2 expressed in E.Coli

Target

MKate2

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

ICC, IHC, WB

Field of Research

Anti-Tag Antibodies

Concentration

200 μg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

26 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

IHC Dilution

1:100-1:500

WB Dilution

1:1000-1:3000

Specificity

Antibody recognizes mKate2, mRuby, mTagBFP2, TurboRFP, mCardinal, and TagRFP-T owing to them all being derived from same fluorescent protein.

ICC Dilution

1:100-1:500

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.