Polyhistidine Anti-6XHIS (for Westerns) Antibody

Our anti-6XHIS antibody recognizes the 6X-HIS tag with high titre and high specificity in western blot applications.

Product Specifications

Background

The synthetic peptide 6X-Histidine (HHHHHH) .

Host

Chicken

Immunogen

Synthetic peptide

Target

6XHIS

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

Field of Research

Anti-Tag Antibodies

Concentration

10 mg/mL

Format

Mixture of IgY fraction and affinity-purified antibodies

Buffer

Sodium phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS, " pH 7.2) (50% by volume) with glycerol (50% by volume) as an anti-freezing agent and sodium azide (0.02%, w/v) as a preservative

Shipping Conditions

Ice packs

Storage Conditions

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Notes

Chickens were immunized with the 6X-Histidine peptide conjugated to keyhole limpet hemocyanin (KLH) and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 μg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

WB Dilution

1:5000-1:10000

Quality Control

This antibody preparation was analyzed by western blotting (at a dilution of 1:10000) using tissue extracts expressing a recombinant protein tagged with the 6X-HIS markerSecondary antibodies were HRP-labeled affinity-purified goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004) .