Anti-Green Fluorescent Protein (GFP) Antibody

Our Anti-Green Fluorescent Protein (GFP) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a Green Fluorescent Protein (GFP), and is mixture of IgY fraction and affinity-purified antibodies. It is great for use in ELISA, IHC, ICC, WB.

Product Specifications

Background

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.

Host

Chicken

Immunogen

Recombinant GFP expressed in Escherichia coli

Target

Green Fluorescent Protein (GFP)

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

ELISA, IHC, ICC, WB

Field of Research

Anti-Tag Antibodies

Concentration

10 mg/mL

Format

Mixture of IgY fraction and affinity-purified antibodies

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Molecular Weight

27 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Notes

Chickens were immunized with recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (30 μg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

IHC Dilution

1:2000-1:5000

WB Dilution

1:5000-1:10000

ICC Dilution

1:2000-1:5000

Quality Control

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.