Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

Our Anti-Glial Fibrillary Acidic Protein (GFAP) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Glial Fibrillary Acidic Protein (GFAP), and is IgY fraction. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Human Glial Fibrillary Acidic Protein (GFAP) is a 49,749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander’s disease, one of the leukodystrophies.

Synonyms

Glial fibrillary acidic protein, GFAP

Gene Name

GFAP

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Recombinant human GFAP expressed in Escherichia coli

Target

Glial Fibrillary Acidic Protein (GFAP)

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

IHC, ICC, WB

Field of Research

Cell Markers / Glial and Schwann Cell Markers

Concentration

2 mg/mL (based on absorbance at 280 nm)

Format

IgY Fraction

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative

Molecular Weight

50 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Antibodies were prepared by injecting laying hens first with highly purified recombinant human GFAP (produced in bacteria), followed by boosts of native GFAP protein purified from bovine spinal cords. After a series of boosts, eggs were collected from hyperimmunized animals and the IgY fraction prepared.

IHC Dilution

1:500-1:1000

WB Dilution

1:1000-1:2000

ICC Dilution

1:500-1:1000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.