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Anti-Glutamic Acid Decarboxylase-67 (GAD 67) Antibody

This affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (CAA01912) and human (NP_000808) gene products.

Product Specifications

Background

Human Glutamic Acid Decarboxylase (GAD-67), [EC 4.1.1.15] is a 66,987 dalton protein (594 amino acids) selectively expressed in a subpopulation of GABAergic neurons of the CNS. It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter beta-amino butyric acid (GABA) . It is also know as GAD-1.

Synonyms

Glutamate decarboxylase 1. EC 4.1.1.15, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform

Gene Name

GAD1 GAD GAD67

UniProt

Q99259

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide

Target

Glutamic Acid Decarboxylase-67 (GAD 67)

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

IHC, ICC, WB

Field of Research

Cell Markers / Neuronal Markers

Concentration

100 μg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

67 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the Cterminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, the concentrations of the eluate adjusted to 100 μg/mL, and the preparation was filter-sterilized through a 0.45 μm filter.

IHC Dilution

1:500-1:1000

WB Dilution

1:1000-1:2000

ICC Dilution

1:500-1:1000

Quality Control

This antibody preparation was analyzed by immunohisto-chemistry (at a concentration of 1 μg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human, Mouse, Rat

Conjugate

Unconjugated

RRID

AB_2313545

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