Anti-C-MYC Epitope Tag Antibody

This product is an unlabeled, affinity-purified chicken anti-C-MYC epitope antibody.

Product Specifications

Background

The synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) .

Synonyms

CMyc, c-Myc, Myc

Host

Chicken

Immunogen

Synthetic peptide

Target

C-MYC Epitope Tag Antibody

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

ELISA, IHC, ICC, IP, WB

Field of Research

Anti-Tag Antibodies

Concentration

100 μg/mL (based on absorbance at 280 nm)

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin (KLH) . After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 μg/mL, and the preparation was filter-sterilized.

IHC Dilution

1:100-1:250

WB Dilution

1:500-1:1000

ICC Dilution

1:100-1:250

Quality Control

Antibodies were analyzed using ELISA and western blots using an N-terminal FLAG®-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.