What is the catalog number for Anti-HA Epitope Tag Antibody?

The catalog number for Anti-HA Epitope Tag Antibody is ET-HA100.

What sizes are available for Anti-HA Epitope Tag Antibody?

Anti-HA Epitope Tag Antibody is available in the following size(s): 1 mL.

Is Anti-HA Epitope Tag Antibody in stock?

Anti-HA Epitope Tag Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-HA Epitope Tag Antibody

Our Anti-HA Epitope Tag Antibody chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects HA Epitope Tag Antibody, and is affinity-purified IgY. It is great for use in ELISA, IHC, ICC, IP, WB.

Product Specifications

Background

The synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA) .

Synonyms

HA, HA Tag, HA Epitope Tag, Hemagglutinin

Host

Chicken

Immunogen

Synthetic peptide

Target

HA Epitope Tag Antibody

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

ELISA, IHC, ICC, IP, WB

Field of Research

Anti-Tag Antibodies

Concentration

100 μg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Chickens were immunized with a synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA) conjugated to keyhole limpet hemocyanin (KLH) . After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 μg/mL, and the preparation was filter-sterilized.

IHC Dilution

1:500

WB Dilution

1:5000

ICC Dilution

1:500

Quality Control

Antibodies were analyzed using ELISA and western blots using an N-terminal HA-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.