Anti-Coronin 1A Antibody

This affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034028) and human (NP_009005) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 μg/mL. This antibody has been validated with human, mouse and rat tissues.

Product Specifications

Background

Human Coronin 1a is a 51,026 dalton protein (461 amino acids) selectively expressed in cells involved in immune surveillance throughout the body, including activated microglial cells of the CNS. It is a member of the WD40 (tryptophan-aspartate) gene family, which includes the beta subunit of the trimeric G proteins as the prototype. Its function is presumably in membrane structural reorganization accompanying antigen-presentation.

Synonyms

Coronin-1A, Coronin-like protein A, Clipin-A, Coronin-like protein p57, Tryptophan aspartate-containing coat protein, TACO

Gene Name

CORO1A CORO1

UniProt

P31146

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide

Target

Coronin 1a

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

IHC, ICC, WB

Field of Research

Cell Markers / Glial and Schwann Cell Markers

Concentration

100 μg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

51 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a sequence shared between the human (NM_007074) and mouse (NM_009898) gene products. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 μg/mL, and the preparation was filter-sterilized.

IHC Dilution

1:1000-1:2000

WB Dilution

1:5000-1:10000

ICC Dilution

1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.