Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody

Our Beta-Galactosidase antibody recognizes the LacZ gene product with high titre and high specificity. Since this antibody was generated in chickens, it can be used together with your existing rabbit and mouse primary antibodies for double immunostaining with no secondary antibody cross reactivity. It even works well with fusion proteins of LacZ!

Product Specifications

Background

Beta Galactosidase is the product of the LacZ gene and is widely used as a reporter marker to monitor gene expression.

Synonyms

Beta-Gal, Beta-Galactosidase, LacZ

Host

Chicken

Immunogen

Native protein expressed Escherichia coli

Target

Beta-Gal (Beta-Galactosidase, LacZ)

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

IHC, ICC, WB

Field of Research

Anti-Tag Antibodies

Concentration

10 mg/mL

Format

Mixture of IgY fraction and affinity-purified antibodies

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with thimerosal (0.01%, w/v) as an anti-microbial agent.

Molecular Weight

117 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Notes

Chickens were immunized with purified ß-Galactosidase (LacZ) protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using ß-Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 μg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with thimerosal and then filter-sterilized.

IHC Dilution

1:2500-1:5000

WB Dilution

1:5000-1:10000

ICC Dilution

1:2500-1:5000

Quality Control

This antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:5000) using tissues infected with a virus bearing the LacZ gene. Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1:2000 dilution, Aves Labs Cat.# F-1005) .