Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody

Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.

Product Specifications

Background

Purified actin (42 kDa) isolated from rabbit muscle

UniProt

P63267

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Actin purified from Rabbit muscle

Target

Actin

Clonality

Polyclonal

Isotype

IgY

Type

Primary Antibody

Applications

Concentration

10 mg/mL

Format

Mixture of IgY fraction and affinity-purified antibodies

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Molecular Weight

42 kDa

Shipping Conditions

Ice packs

Storage Conditions

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Notes

Chickens were immunized with purified actin from rabbit muscle emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using actin conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 μg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

WB Dilution

1:1,000-1:2,000

Quality Control

This anti-actin IgY preparation was analyzed by western blot analysis (at a dilution of 1:2000) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004) as the secondary reagent.