Anti-Beta-Amyloid Peptide (N-terminus) Antibody

Beta-Amyloid peptide is a 40- or 42-amino acid fragment of the human Beta-Amyloid Peptide: Precursor Protein (770 amino acids) produced by the proteolytic actions of Beta and Gamma-secretases. Both forms of Beta-Amyloid peptide are rather insoluble and tend to self-aggregate into distinctive extracellular "plaques." These plaques are evident in brains from patients with Alzheimer's disease as well as in brains from individuals with a history of traumatic head injuries. In the case of Alzheimer's disease, it has been suggested that these extracellular Beta-Amyloid peptide plaques are themselves cytotoxic (rather than simply being markers of brain pathology), and are responsible for the dendritic pruning and other neurodegenerative changes seen.

Amyloid-beta A4 protein (ABPP) (APPI) (APP) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Amyloid-beta precursor protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha) ; Soluble APP-beta (S-APP-beta) ; C99 (Beta-secretase C-terminal fragment) (Beta-CTF) ; Amyloid-beta protein 42 (Abeta42) (Beta-APP42) ; Amyloid-beta protein 40 (Abeta40) (Beta-APP40) ; C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF) ; P3 (42) ; P3 (40) ; C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID (59) ) (Gamma-CTF (59) ) ; Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID (57) ) (Gamma-CTF (57) ) ; Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID (50) ) (Gamma-CTF (50) ) ; C31]

Synthetic peptide (DAE FRH DSG YEV HHQ KL) contained within both the 40- and 42 amino acid versions of the human Beta-Amyloid peptide (Amyloid Precursor Protein residues #672-688) .

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Chickens were immunized with a synthetic peptide (corresponding to DAE FRH DSG YEV HHQ KL, residues #1-17 of the amyloid beta-peptide) coupled to keyhole limpet hemocyanin (KLH) . After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached. Finally, the antibody concentration was adjusted to 100 ug/ml and filter-sterilized.

Antibodies were analyzed by immunohistochemistry (at a concentration of 3 μg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) or HRP-labeled goat anti-chicken IgY (1:2000 dilution, Aves Labs, Cat.# H-1004) as the secondary reagent.