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Anti-Cav1.2 Ca2+ Channel (N500/4) Recombinant Chicken Chimeric mAb

Our chicken Anti-Cav1.2 Ca2+ Channel recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N500/4. It detects human, mouse, and rat Cav1.2 Ca2+ Channel and is purified by affinity chromatography. It works in ELISA, ICC, IHC, WB.

Product Specifications

Background

Anti-Cav1.2 Ca2+ Channel Recombinant Chicken Chimeric mAb | 78-535

Synonyms

Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c

Gene Name

Cacna1c

UniProt

Q13936

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Recombinant protein amino acids 785-900 (ID I-II cytoplasmic loop) of mouse Cav1.2 (also known as voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c, accession number Q01815) produced recombinantly in E. Coli.

Target

Anti-Cav1.2 Ca2+ Channel Recombinant Chicken Chimeric mAb | 78-535

Clonality

Recombinant

Isotype

IgY

Clone

N500/4

Type

Primary Antibodies

Applications

ELISA, ICC, IHC, WB

Field of Research

Cardiovascular / Ion Channels and Modulators / Ca2+ Channels

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

pH

7.4

Molecular Weight

240 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N500/4 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N500/4 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

IHC Dilution

1:100-1:500

WB Dilution

1:500-1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:100-1:500

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.

Brand

Aves Labs

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

WB Brain Dilution

1:500-1:1000

Conjugate

Unconjugated

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