Anti-Ryanodine Receptor Type 2 (RyR2) (N501/49) Recombinant Chicken Chimeric mAb

Our chicken Anti-RyR2 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N501/49. It detects human, mouse, and rat RyR2 and is purified by affinity chromatography. It works in ELISA, ICC, IHC, WB.

Product Specifications

Background

Anti-RyR2 Recombinant Chicken Chimeric mAb | 78-534

Synonyms

RyR2, Ryr2, RYR-2

Gene Name

Ryr2

UniProt

Q92736

Host

Chicken

Species Reactivity

Human,Mouse,Rat

Immunogen

Recombinant protein amino acids 2672-2835 (cytoplasmic) of mouse Ryanodine Receptor Type 2 (also known as RyR2, Ryr2, accession number E9Q401) produced recombinantly in E. Coli.

Target

Anti-Ryanodine Receptor Type 2 (RyR2) Recombinant Chicken Chimeric mAb | 78-534

Clonality

Recombinant

Isotype

IgY

Clone

N501/49

Type

Primary Antibodies

Applications

ELISA, ICC, IHC, WB

Field of Research

Cardiovascular / Ion Channels and Modulators / Ca2+ Channels / Neurodegenerative Disease / Alzheimer's Disease

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

pH

7.4

Molecular Weight

564 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N501/49 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N501/49 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

IHC Dilution

1:100-1:500

WB Dilution

1:500-1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:100-1:500

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.