Anti-Phospho-GUK1 (Tyr74) Antibody

Guanylate kinase 1 (GUK1) is an essential enzyme in the purine salvage pathway, catalyzing the reversible conversion of GMP to GDP. This reaction is a rate-limiting step in maintaining the cellular GTP pool required for nucleic acid synthesis, signal transduction, and energy metabolism. Clinically, GUK1 is vital for the metabolic activation of guanine-based prodrugs, making it a determinant of antiviral and chemotherapeutic efficacy. GUK1 phosphorylation at Tyr74 by non-receptor tyrosine kinases (e.g., Src or Syk) has been linked to increased nucleotide production in hyper-proliferative states. Elevated levels of Tyr74-phosphorylated GUK1 are frequently observed in various malignancies, where they support the metabolic demands of rapid tumor growth, positioning this specific phospho-form as a significant biomarker for metabolic reprogramming in oncology and drug resistance studies. It is phosphorylated by the fusion kinase EML4-ALK in lung cancers, creating a metabolic dependency, and making it a potential target for therapy.

Our GUK1 (Tyr74) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat GUK1 (Tyr74) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, ELISA.